10.
IMMUNOLOGICAL
AND SEROLOGICAL REAGENTS
Major
breakthroughs in microbiology and for that matter in biology since
1960 have been in the field of immunology and serology.
Concepts and skills, especially methodology, of these important
areas are, therefore, essential to providing a complete and up-to-date
picture of microbiology and biology. Many of these techniques also
form the basis of biotechnology and genetic engineering making their
learning even more important.
Unfortunately,
reagents used in these areas are produced for use by clinical
laboratories and, therefore, are not only expensive, but are often
hard to find. An answer
to this dilemma is classroom quality immunological reagents, which are
relatively inexpensive. This
catalogue lists such reagents and thus opens ways for instructors to
teach a variety of antigen-antibody reactions, such as the precipitin
reaction, the agglutination reaction, the complement fixation
reaction, the neutralization reaction, the hem agglutination reaction,
the passive hem agglutination reaction, immunodouble diffusion or the
Ouchterlony reaction and immunoelectrophoresis. Simultaneously, an instructor can also show how these tests
are used in the design of serological tests for the diagnosis of
disease, for testing the purity of proteins and other antigens and in
medico legal areas such as forensics.
In
addition to providing serological reagents suitable for teaching, the
Institute is also available to assist you in the design and
implementation of immunological and serological experiments for your
classes. So, feel free to
call the Institute with your questions and comments.
DETAILED
LABORATORY HANDOUTS explaining the principles and procedures of
immunological and serological reactions are also available from the
Institute. For price
information, please refer to the end of this section.
REAGENTS
FOR AGGLUTINATION REACTION - with directions
Catalogue
#
Description
Qty
Price
IM-01
Killed, standardized suspension of Salmonella typhosa
5.0 ml $5.00
IM-02
Killed, standardized suspension of Salmonella Montevideo
5.0 ml
$5.00
IM-03
Suspension of Salmonella typhosa marked as unknown #1
5.0 ml $5.00
IM-04
Suspension of Salmonella montevideo
marked as unknown #2 5.0 ml
$5.00
IM-05
Suspension of Salmonella cholerae-suis marked as unknown #3 5.0 ml
$5.00
(Use
of these suspensions as unknowns enables students to understand the
usefulness of agglutination reactions and, also, to test their ability
to read and differentiate between a positive and a negative
agglutination reaction.)
IM-06
Anti Salmonella typhosa
5.0 ml
$12.50
IM-07
Anti Salmonella Montevideo
5.0 ml
$12.50
IM-08
Anti Salmonella cholerae-suis
5.0 ml
$12.50
REAGENTS FOR PRECIPION REACTION ALSO FOR
IMMUNODOUBLE DIFFUSION, ELECTROPHORESIS
AND IMMUNOELECTROPHORESIS - with directions
Catalogue #
Description Qty.
Price
IM-09
Human serum
5.0 ml
$12.50
IM-10
Goat serum
5.0 ml
$7.50
IM-11
Horse serum
5.0 ml
$7.50
Catalogue #
Description
Qty.
Price
IM-12
Human gamma globulin
5.0 ml $20.00
IM-13
Human albumin
5.0 ml
$10.00
IM-14
Anti human serum
5.0 ml
$20.00
IM-15
Anti goat serum
5.0 ml
$15.00
IM-16
Anti horse serum
5.0 ml
$15.00
NOTE: For supplies for Ouchterloney double diffusion,
electrophoresis and immuno-electrophoresis, refer to the Section on
Special supplies and reagents for Immunological reactions.
REAGENTS FOR NEUTRALIZATION REACTION
a)
Bacteriophage
and antibacteriophage system
- with directions
Catalogue
#
Description
Qty.
Price
VL-01
Suspension of E. coli T4 bacteriophage
2.0
ml $10.00
IM-11
Anti E. coli T4 bacteriophage
5.0 ml $10.00
LB-33
E. coli strain B
$5.00
Toxin and antitoxin system - with directions
Catalogue
#
Description
Qty.
Price
IM-18
Bacterial filtrate containing hemolytic factors
3.0 ml
$15.00
IM-19
Antihemolytic factors
3.0
ml
$20.00
Blood
plate for hemolytic reaction
$1.00
REAGENTS FOR PASSIVE HEMAGGLUTINATION
REACTION - with directions
Catalogue
#
Description
Qty.
Price
IM-20
Soluble antigen coupled to red blood cells
5.0 ml
$25.00
IM-21
Red blood cells without soluble antigen
5.0 ml
$10.00
IM-22
Anti soluble antigen
5.0 ml $15.00
IM-23
Soluble antigen solution
5.0 ml $20.00
REAGENTS FOR COMPLEMENT FIXATION
REACTION - sufficient for 30 students - with directions
Catalogue
#
Description
Qty.
Price
IM-24
Antigen suspension
3.0 ml
$5.00
IM-25
Positive control serum for complement fixation
5.0 ml $15.00
IM-26
Negative control serum for complement fixation
5.0 ml $10.00
IM-27
Unknown serum - for complement fixation
5.0 ml $15.00
IM-28
Complement - for complement fixation
5.0 ml $25.00
IM-29
Sheep blood for complement fixation
10.0 ml $15.00
IM-30
Anti-sheep red blood cells for complement fixation
3.0 ml $15.00
IM-31
Veronal Buffer for complement fixation
250 ml $10.00
11.
REAGENTS
FOR BLOOD TYPING
IM-32
Anti-A serum
5.0 ml $7.50
IM-33
Anti-B serum
5.0 ml
$7.50
IM-34
Anti-Rh serum
5.0 ml
$20.00