International Institute of Biochemical and Biomedical Technology

International Institute of Biochemical and
Biomedical Technology

Green Microbiology Workshop

Catalog of Microbiological and Immunological Supplies

Click here to view a printable version of the catalog.

CONTENTS

1. THE PURPOSE OF THIS CATALOGUE
2. WHY REVIVE CLASSICAL MICROBIOLOGY
3. HOW TO REVIVE CLASSICAL MICROBIOLOGY
4. ADVANTAGES OF USING THIS CATALOGUE
5. ORDERING INFORMATION
6. INTRODUCTION TO MAJOR GROUPS OF MICROORGANISMS (KITS)
7. CULTURES - LIVE OR KILLED:

Bacteria
Actinomycetes
Fungi (molds and yeasts)
Viruses (bacterial)

8. MEDIA - PREPARED, for

Bacteria
Fungi
Actinomycetes

9. ANTIBIOTIC DISCS FOR SENSITIVITY TESTING
10. IMMUNOLOGICAL AND SEROLOGICAL REAGENTS FOR:

Agglutination reaction
Precipitation reaction
Neutralization reaction
Viral and toxin-antitoxin systems
Passive Hemagglutination reaction
Complement fixation reaction

11. BLOOD TYPING REAGENTS
12. SYPHILIS TEST REAGENTS
13. SPECIAL SUPPLIES AND REAGENTS for immunological reactions
14. SLIDES - PREPARED

Bacteria
Fungal
Actinomycetes

15. STAINS AND STAINING REAGENTS FOR:

Acid fast stain
Capsule stain
Cell wall stain
Cytoplasmic membrane stain
Gram stain
Fat droplet stain
Flagella stain
Metachromatic granule stain
Nuclear material stain
Spore stain
Simple and negative stains
Fungal stain

16. BIOCHEMICAL TEST REAGENTS FOR:

Bacitracin sensitivity test for Group A streptococci
Bile solubility test
CAMP test
Catalase test
Coagulase test
DNAase test
Gelatin hydrolysis test
Gluconaate hydrolysis test
Hippurate hydrolysis test
Indole test
Methyl red test
Nitrate reduction test
Optochin test for pneumococci
Oxidase test
Phenylalanine deaminase test
Phosphatase test
Starch hydrolysis test
Voges-Proskauer test

17. RAPID AND MULTI-TEST IDENTIFICATION SYSTEMS
18. LAB ESSENTIALS - MATERIALS AND SUPPLIES
19. THE LABORATORY MANUAL - A CURRICULUM OF BASIC AND APPLIED MICROBIOLOGY

1. THE PURPOSE OF THIS CATALOGUE
     The purpose of this catalogue is to help revive classical microbiology - a subject that should never have been treated as a specialty subject but as a subject, which must be known and understood by everyone.

2. WHY REVIVE CLASSICAL MICROBIOLOGY
     Microbiology is an exciting way of introducing students to science. It is also a way of orienting students towards a variety of careers. Medical and para-medical professions are the obvious ones but microbiology is also essential for environmental sciences such as water quality and waste management, pollution studies and abatement, management of toxic wastes, and agriculture, especially alternate methods of farming based on natural versus chemical farming. Microbiology is also essential for biotechnology, genetic engineering, fermentation, food and pharmaceutical industries, and space exploration.

     Microbiology is also essential for understanding the economy of nature and the balanced way in which nature functions. As such it has direct application in the areas of economics and in developing sound economic institutions and policies - an application hitherto unexplored. This aspect of microbiology is especially useful now when we need to design interdependent, perpetual, productive and efficient economic systems, which could not only sustain but also aid in improving the quality of life of expanding human population.

     There is no better place to learn and see principles of such systems in operation than in microbiology where an organism divides into two before it becomes inefficient, where small is sustaining, not only itself but the rest of the pyramid of life upto its very peak. It is this knowledge, which must go into the making of our economists, philosophers, and thinkers. Unfortunately, economic majors neither necessarily study biology nor microbiology. How can we expect them to create viable economic systems if we never exposed them to the only known perpetuating system which has existed from the beginning of time and which still goes on despite all the follies of humankind. A revival of classical microbiology with its philosophical implications should thus be a priority for teachers, administrators and planners and they should see to it that this knowledge becomes part of everyone's general knowledge.

     Microbiology is also essential for personal hygiene and disease prevention. It was the knowledge of microbiology that, upon reaching the public via newspapers, enhanced standards of living and brought infectious diseases under control. Only when we took this knowledge out of public awareness that the diseases began to surface again. The public fought polio more than the established government and academic institutions. Same can be done for Aids but for that, teaching of classical microbiology has to be revived and it should, once again, be made part of everyone's knowingness.

     Too many colleges and universities are now giving microbiology the back seat. In many cases, even though the departments are named "Microbiology," their course offerings and the thrust of their educational direction is no way near the classical discipline of microbiology. This, infact is a disservice for the public and even the planners at the national and international level keep on believing that microbiology is alive and well. The reality surfaces only when they need a microbiologist and learn that the one who carried the credentials was not a functioning microbiologist.

     This trend needs to be changed which is partly due to funding priorities forcing microbiologists to branch out into other areas yet continue to wear their original hats. Unfortunately, as time goes on, their newfound interests begin to shape their course contents at the expense of classical microbiology. Their students in turn reflect their own interests in the courses they offer, making each successive generation farther and farther removed from classical microbiology.

     This shift in funding priorities, coupled with lack of appreciating the importance of classical microbiology, led many institutions, in the 70's, to make microbiology a lecture and demonstration course while to know this subject well, one needs to go through a hands-on curriculum which is lab based. No one can fully grasp the impact of microbiology unless they have seen living microorganisms under a phase contrast microscope. That is one of the reasons why Zernick was given the Nobel prize in 1935 for inventing this microscope. How many educational institutions are using this educational tool to open up the world of microorganisms to their students? You may be thinking that this will be an expensive proposition but in reality it is not so, especially when phase microscopes are not as expensive now as they once used to be and also when all one needs is one phase microscope per class and even that can be shared with other classes. The fact is that curriculum designers of today are not familiar with the educational value of such tools. This again casts a poor reflection on the educational institutions and their faculty who failed to appreciate the importance of their basic discipline and thereby failed to ensure its perpetuity. The damage in this area is far too severe which can be easily assessed by realizing that there exist, in this nation of ours, professional schools where microbiology is no longer taught to students of nursing and to students of pharmacy. Need I say more. That is why we have to revive classical microbiology and we have to do it now.

3. HOW TO REVIVE CLASSICAL MICROBIOLOGY
     The best way to revive classical microbiology is to facilitate hands-on lab-based teaching of microbiology. Here, the most common compliant is that the essential materials and supplies are either too expensive or are not available. Most lab., supply houses, which once used to cater to schools and colleges, are now concentrating on supplying to hospitals, clinical laboratories, industries and research institutions mainly because that is where the money is.

     Under these circumstances, the way to revive classical microbiology is to make available needed microbiological supplies and also to provide any guidance the institutions and the instructors may need for teaching this subject. It is for this reason that this mini-catalogue of microbiological supplies was put together. It contains only the essentials needed to teach a hands-on lab-based course in microbiology and to do so inexpensively. The supplies contained in this catalogue are designed for classroom use only and are not intended for clinical or other testing laboratories or for research.

4. ADVANTAGES OF USING THIS CATALOGUE
     This catalogue provides you everything that you need to teach a good "hands on" course in microbiology in one place. Nothing extra to hunt for or to buy. By using this catalogue you can teach your students a comprehensive course in microbiology covering such topics as:

An introduction to the ten major groups of microorganisms.
Sampling techniques and the isolation and cultivation of microorganisms.
Pure culture techniques in microbiology.
Cytological, biochemical and serological procedures.
Identification and quantitation of microorganisms.
Immunological procedures.
Microbial genetics.
Industrial microbiology procedures.
Sanitary and environmental microbiology procedures.
Clinical microbiology procedures.
Mycological procedures
Virological procedures - propagation and quantitation of viruses.

     All items included in this catalogue are GUARANTEED CLASS ROOM QUALITY MATERIALS and are provided in standardized form in sufficient quantities to suffice for a class of up to 30 students.
     A COMPLETE OBJECTIVE ORIENTED LABORATORY MANUAL covering the entire basic and applied field of microbiology is also available (see details on page 31 of this catalogue). This manual together with this catalogue is an ideal combination for you to update your microbiology courses and to make them exciting learning experiences for your students.
     The use of the manual, however, is not mandatory, for the catalogue will compliment any laboratory manual that you may now be using or wish to use.
     Please also note that since we specialize in providing only microbiological and immunological supplies through this catalogue, we can assure you prompt deliveries with almost no back orders. You will also like our low prices and when you call us you will find a knowledgeable person on the other end who can not only answer your questions but can also consult with you intelligently. So, please keep our phone and fax numbers handy.

Phone: (312) 243-2016; FAX: (312) 243-2041; E-mail: iibbt@iibbt.com

5. ORDERING INFORMATION
     We provide supplies only to bonafide institutes of learning.

     All items in this catalogue are Guaranteed Classroom Quality®. They are intended for teaching purposes only and are not to be used for clinical or other testing purposes.

     How to place an order:
     Using your official purchase order form or letterhead, please supply the following information about each item ordered:

Name of the product
Catalogue number
Size
Quantity desired
Date delivery required
Price

     Additionally, please provide your purchase order number and shipping and billing addresses and mail orders to:
     Educational Materials Division
     Int. Inst. of Biochemical and Biomedical Technology
     731 South Western Avenue
     Chicago, Illinois, USA 60612-3532
     For expediency, place orders by FAX: (312) 243-2041.
     We do not accept phone orders unless followed by an official faxed or mailed hard copy.

     Credit
     Credit is extended to all educational institutions who send their orders on their official purchase order form or letterhead and signed by an authorized individual from the institute. Such orders will be honored and shipped promptly. Payment for these orders is due when billed.
     All other institutions and individuals are required to enclose payment with their order. Please include 15% shipping and handling costs with your payment.

     Prices
     Prices are f.o.b. our address and are subject to change without notice. All billing is done at current prices plus shipping and handling charges.

     Guarantee
     Our products are Guaranteed Classroom Quality® and they are unconditionally guaranteed. Items found unsatisfactory will be replaced. Please notify our Educational Materials Division before returning such items. We take used equipment as trade in.

6. INTRODUCTION TO MAJOR GROUPS OF MICROORGANISMS (KITS)

     A. MATERIALS FOR INTRODUCING STUDENTS TO THE MAJOR GROUPS OF MICROORGANISMS

     The vastness of microbiology can be overwhelming to a student especially when we, as teachers, emphasize that microorganisms constitute a large group of diverse organisms. A beginning student is not able to relate to such a generalized statement. He wishes to have a sense of reference and a way to relate to the vast number of organisms.      One of the ways to give such a sense of reference is to introduce the students to the major groups of microorganisms. Generally, microorganisms are placed in anywhere from 10 to 12 major groups. These are: algae, protozoa, fungi, actinomycetes, blue green algae, bacteria, mycoplasma, L forms, Rickettsia, Chlamydia, slime molds, and viruses. Kits listed below provide related live and/or preserved materials to introduce students to all of the above groups of microorganism. With the help of these materials you will be able to show the students actual organisms and/or their colonies rather than just talk about them. As we all know, there is no substitute for learning by seeing and doing.

     B. MATERIAL FOR INTRODUCING STUDENTS TO HIGHER PROTISTA

     This material is supplied in a kit form and includes selected live and/or preserved material for introducing students to algae, protozoa, slime molds and fungi. Material is sufficient for a class of up to 30 students.

     Catalogue # HP-1 Higher Protista Kit   $75.00

     C. MATERIAL FOR INTRODUCING STUDENTS TO LOWER PROTISTA

     This material is supplied in a kit form and includes selected live and/or preserved material for introducing students to blue green algae, bacteria, actinomycetes, mycoplasma or L forms, rickettsia, chlamydia and viruses. Material is sufficient for a class of up to 30 students.

     Catalogue # LP-2 Lower Protista Kit   $75.00

     D. FREE CONSULTATION

     If you are designing a new course or are updating an old one - call us. We can enable you to offer a very comprehensive lab course at much less of a cost than you can imagine.

Phone: (312) 243-2016
FAX: (312) 243-2041
e-mail: iibbt@iibbt.com

7. CULTURES
     While there is an overwhelming number of cultures, one needs only a few, in most cases nonpathogenic ones, to teach the essential principles of microbiology. Nonetheless, the list on the following pages is rather extensive and is provided so that different instructors can select cultures of their choice.

     We would, however, like to emphasize that keeping too many unnecessary cultures in a teaching laboratory is time consuming as they need to be transferred frequently and also their purity has to be tested at frequent intervals. This time can be saved by keeping only the essential cultures and limiting one's stock of cultures to the hardier ones rather than those which die rapidly when maintained in the laboratory.

     It should also be noted that live cultures are not always necessary for teaching principles of microbiology. Procedures designed to demonstrate morphology and staining reactions such as the Gram stain and cytological stains can be performed on killed cultures. Live cultures are essential only for biochemical reactions and for growth experiments. Immunological and serological reactions also do not require live cultures. Killed cultures are the cultures of choice here, and this catalogue provides such cultures. These are easy to keep and since they have a longer shelf life, they are economical to use. A considerable amount of cost can thus be saved by using the killed cultures as opposed to live cultures.

     The use of the killed cultures also enables the students and staff to be less apprehensive about working with microbes, especially since the connotation "germs" is associated with disease production. Such cultures would, therefore, enable you to add lab based microbiology courses at introductory college or even at high school level. We take used equipment as trade in. Write or call for details.

Phone: (312) 243-2016
FAX: (312) 243-2041
e-mail: iibbt@iibbt.com

     NOTE: We regard most living cultures as potentially pathogenic and ship these only to colleges and universities. Cultures which we ship to high schools are marked with an asterisk. We do not ship cultures to individuals.

     All bacterial and yeast cultures are available as live cultures on slants and also as killed suspensions.

     Price per live culture is $5.00 plus shipping and handling.

     Price per 5.0 ml of killed suspension is $7.50 plus shipping and handling.

     All fungal and actinomycetes cultures are available as live cultures on slants and also as killed mycelial mass. The latter is prestained, ready to tease, mount, and observe.

     Live fungal or actinomycetes cultures are $7.50 each.

     Preserved mycelial mass is $20.00. The amount is sufficient for a class of 30 students.

     Bacterial viruses are supplied as live suspensions. Price per 2.0 ml vial is $10.00.

CULTURES OF BACTERIA - LIVE OR KILLED

Catalogue #
Live	Killed	Description
LB-01	KB-01	Aeromonas hydrophilia
LB-02	KB-02	Alcaligenes faecalis
LB-03	KB-03	Alcaligenes viscolactis
LB-04	KB-04	Arizona hinshawii
LB-05	KB-05	Bacillus alvei
LB-06	KB-06	Bacillus cereus*
LB-07	KB-07	Bacillus cereus var. mycoides
LB-08	KB-08	Bacillus coagulans
LB-09	KB-09	Bacillus megaterium*
LB-10	KB-10	Bacillus polymyxa
LB-11	KB-11	Bacillus stearothermophilus
LB-12	KB-12	Bacillus subtilis*
LB-13	KB-13	Bacillus subtilis var. globigii
LB-14	KB-14	Bacteroides fragilis
LB-15	KB-15	Branhamella catarrhalis (previously Neisseria catarrhalis)
LB-16	KB-16	Chromobacterium violaceum
LB-17	KB-17	Citrobacter freundii (previously Escherichia freundii)
LB-18	KB-18	Citrobacter intermedius
LB-19	KB-19	Clostridium botulinum
LB-20	KB-20	Clostridium butyricum
LB-21	KB-21	Clostridium difficile
LB-22	KB-22	Clostridium histolyticum
LB-23	KB-23	Clostridium perfringens
LB-24	KB-24	Clostridium sporogenes
LB-25	KB-25	Clostridium tetani
LB-26	KB-26	Corynebacterium diphtheriae
LB-27	KB-27	Corynebacterium pseudodiphtheriticum*
LB-28	KB-28	Corynebacterium xerosis
LB-29	KB-29	Edwardsiella tarda
LB-30	KB-30	Enterobacter aerogenes (previously Aerobacter aerogenes)
LB-31	KB-31	Enterobacter cloacae
LB-32	KB-32	Escherichia coli*
LB-33	KB-33	Escherichia coli Strain B*
LB-34	KB-34	Escherichia coli (gene transfer donor)
LB-35	KB-35	Escherichia coli (gene transfer recipient)
LB-36	KB-36	Flavobacterium capsulatum*
LB-37	KB-37	Gaffkya tetragena
LB-38	KB-38	Hafnia alvei
LB-39	KB-39	Halobacterium salinarium
LB-40	KB-40	Klebsiella pneumoniae
LB-41	KB-41	Lactobacillus acidophilus
LB-42	KB-42	Lactobacillus casei
LB-43	KB-43	Leuconostoc mesenteroides
LB-44	KB-44	Listeria monocytogenes
LB-45	KB-45	Micrococcus luteus* (previously Sarcina lutea)
LB-46	KB-46	Morganella morganii (previously Proteus Morganii)
LB-47	KB-47	Mycobacterium smegmatis
LB-48	KB-48	Mycobacterium phlei*
LB-49	KB-49	Mycobacterium tuberculosis
LB-50	KB-50	Neisseria subflava
LB-51	KB-51	Neisseria sicca
LB-52	KB-52	Photobacterium fischeri
LB-52	KB-53	Proteus mirabilis
LB-53	KB-54	Proteus vulgaris
LB-54	KB-55	Pseudomonas aeruginosa
LB-56	KB-56	Pseudomonas cepacia
LB-57	KB-57	Pseudomonas fluorescens
LB-58	KB-58	Pseudomonas putida
LB-59	KB-59	Providencia rettgeri
LB-60	KB-60	Providencia stuartii
LB-61	KB-61	Rhodococcus rhodochrous
LB-62	KB-62	Rhodospirillum rubrum*
LB-63	KB-63	Salmonella cholerae-suis
LB-64	KB-64	Salmonella enteritidis
LB-65	KB-65	Salmonella montevideo
LB-66	KB-66	Salmonella paratyphi A
LB-67	KB-67	Salmonella gallinarum
LB-68	KB-68	Salmonella schottmuelleri (Salmonella paratyphi B)
LB-69	KB-69	Salmonella typhimurium
LB-70	KB-70	Salmonella typhosa
LB-71	KB-71	Sarcina lutea (see Micrococcus luteus)
LB-72	KB-72	Serratia marcescens*
LB-73	KB-73	Shigella boydii
LB-74	KB-74	Shigella dysenteriae
LB-75	KB-75	Shigella flexneri
LB-76	KB-76	Shigella sonnei
LB-77	KB-77	Spirillum itersonii
LB-78	KB-78	Spirillum serpens*
LB-79	KB-79	Staphylococcus epidermidis*
LB-80	KB-80	Staphylococcus aureus
LB-81	KB-81	Staphylococcus aureus (for CAMP test)
LB-82	KB-82	Staphylococcus saprophiticus*
LB-83	KB-83	Streptococcus agalactiae
LB-84	KB-84	Streptococcus sp., (cariogenic)
LB-85	KB-85	Streptococcus sp., viridans - alpha hemolytic
LB-86	KB-86	Streptococcus sp., ahemolyticus, - gamma hemolytic
LB-87	KB-87	Streptococcus sp., beta hemolytic - group A (S. pyogenes)
LB-88	KB-88	Streptococcus sp., beta hemolytic - group B
LB-89	KB-89	Streptococcus sp., beta hemolytic - group D
LB-90	KB-90	Streptococcus sp., enterococcus, alpha to gamma hemolytic- group D
LB-91	KB-91	Streptococcus faecalis
LB-92	KB-92	Streptococcus faecalis var. liquefaciens
LB-93	KB-93	Streptococcus lactis*
LB-94	KB-94	Streptococcus penumonia (previously pneumococcus pneumoniae)
LB-95	KB-95	Streptococcus salivarius
-------	PS-38	TB positive sputum smears. These are supplied unstained and are prepared from sterilized known positive TB sputum $1.50 ea.

Catalogue #
Live	Killed	Description
-------	KB-95	Vibrio cholera - available only as a killed suspension
LB-96	KB-96	Vibrio fischeri

CULTURES OF ACTINOMYCETES - LIVE OR KILLED
Catalogue #
Live	Killed	Description
LA-98	KA-98	Streptomyces griseus
CULTURES OF FUNGI (MOLDS AND YEAST) - LIVE OR KILLED
Catalogue #
Live	Killed	Description
LA-99	KF-99	Alternaria sp.
LF-100	KF-100	Aspergillus niger*
LF-101	KF-101	Candida albicans
LF-102	KF-102	Candida parapsilosis
LF-103	KF-103	Candida pseudotropicalis
LF-104	KF-104	Candida tropicalis
LF-105	KF-105	Epidermophyton floccosum
LF-106	KF-106	Geotrichum candidum
LF-107	KF-107	Microsporum canis
LF-108	KF-108	Microsporum gypseum
LF-109	KF-109	Mucor genevensis
LF-110	KF-110	Mucor mucedo
LF-111	KF-111	Penicillium camemberti
LF-112	KF-112	Penicillium chrysogenum
LF-113	KF-113	Penicillium notatum*
LF-114	KF-114	Penicillium roqueforti
LF-115	KF-115	Rhizopus nigricans plus strain*
LF-116	KF-116	Rhizopus nigricans minus strain*
LF-117	KF-117	Saccharomyces cerevisiae*
LF-118	KF-116	Saccharomyces ellipsoideus
LF-118	KF-118	Trichophyton rubrum
LF-120	KF-120	Trichophyton mentagrophytes
LF-121	KF-121	Trichophyton tonsurans
LF-122	KF-122	Torula lactosa
LF-123	KF-123	Torula rosea
BACTERIAL VIRUSES - LIVE ONLY
Catalogue #
Live	Killed	Description
VL-01	-------	Escherichia coli T4 phage (use E. coli strain B for propagation)

8. MEDIA - PREPARED

     While you can prepare your own media, a considerable amount of cost and time can be saved by purchasing prepared media provided you limit the purchase of such media to what is actually needed. Even if you prepare your own media, do not prepare more than what is needed. Microbiological media, especially agar plates, have poor shelf life as they not only dry out but also tend to get contaminated with repeated handling.

     For these reasons, this catalogue lists only those media which are essential for teaching a comprehensive course in microbiology. Prepared media are supplied, sterile, in tubes as slants, deeps, and broths and in standard size Petri plates as agar plates.

     Certain media to be used as plates are also bulk packed in two size bottles. One size bottle has sufficient amount of the medium for preparing 15 plates. The other size has sufficient medium to prepare 5 plates. These bulk packs are specially useful for doing viable counts.

     This is an economical way for preparing plates as the bulk packed medium can be melted and poured in plates as needed. If the entire contents of the bottle are not used, the leftover medium can still be saved and re-melted for preparing more plates. While such re-melted medium may not be suitable for performing clinical or other tests, it is perfectly acceptable for teaching purposes.

     Occasionally also, when only a few plates are needed, agar medium supplied as deeps can be melted and poured into plates. For this reason each deep, as supplied, contains sufficient medium to pour one plate.

     Size and description of containers of prepared media

     All broth tubes are supplied as 16 x 100 or 16 x 150 mm screw cap tubes.

     All slants are supplied as 16 x 100 or 16 x 150 mm screw cap tubes.

     All deeps are supplied as 20 x 150 mm screw cap tubes. Deeps contain approximately 18 ml of medium which is sufficient to pour one plate.

     All stab media are solid or semisolid agars. They are supplied in 16 x 100 or 16 x 150 mm screw cap tubes.

     All bulk packed medium is supplied in approximately 250 ml quantity in 500 ml screw capped bottles or in approximately 100 ml quantity in 250 ml screw capped bottles. Melt media in these bottles by immersing them, halfway, in a beaker of water and then bringing the water slowly to a boil. Keep boiling until the medium is fully melted. Cool medium to approximately 45 C and then pour plates.

     This bulk packed solid medium is also useful for doing viable counts. For this purpose, medium packed in 100 ml quantities is preferred as this amount is sufficient to pour 5 plates.

     MICROBIOLOGICAL MEDIA - PRICES

     The following media are supplied sterile in screw cap tubes, bottles, or Petri plates. Minimum order is 10 tubes or plates. Order additional media in increments of 10 also. No minimum order on botttled media. Media marked with an * are special order media at $1.50 each. All other media are priced as follows:

     Regular broth tube or agar slant is $0.60 ea.
     Blood or chocolate agar slant is $1.25 ea..
     Agar deep is $0.75 ea.
     Broth tube with gas vial is $1.25 ea.
     Agar plate is $0.65 ea.
     Agar bottle containing 250 ml of medium is $15.00 ea.
     Agar bottle containing 100 ml of medium is $10.00 ea.
     NOTE: The institute assumes no responsibility for breakage of bottles or tubes while melting agar media contained in them.

     BACTERIOLOGICAL MEDIA

PM-62	Acetamide agar for acetamide hydrolysis *
PM-77	Azide dextrose broth
PM-63	Bile esculin agar slants
PM-01	Blood agar slant
PM-02	Blood agar plate
PM-03	BHI (Brain Heart Infusion) broth
PM-04	BHI agar slant
PM-05	BHI agar plate
PM-06	BHI agar deep
PM-07	BHI agar bottle 250 ml quantity
PM-08	BHI agar bottle 100 ml quantity
PM-09	BHI broth with 6.5% sodium chloride
PM-10	BCP glucose (Brom Cresol Purple) broth with gas vial
PM-11	BCP lactose broth with gas vial
PM-64	BCP Maltose broth with vial
PM-12	BCP sucrose broth with gas vial
PM-13	Brilliant green lactose bile broth with gas vial
PM-14	Chocolate agar plate
PM-15	Chocolate agar slant
PM-16	CTA glucose (Cystine Trypticase Agar) semisolid stab
PM-17	CTA lactose semisolid stab
PM-18	CTA sucrose semisolid stab
PM-19	CTA maltose semisolid stab
PM-65	Decarboxylase broth with arginine
PM-78	Decarboxylase broth with lysine
PM-79	Decarboxylase broth with ornithine
PM-20	Dextrose-starch agar plate
PM-21	DNAse agar plate
PM-22	Egg yolk agar for lecithinase activity
PM-23	Eugone Agar plate*
PM-24	EMB (Eosin Methylene Blue) agar plate
PM-25	Endo broth
PM-80	F M agar for fusobacterium*
PM-26	Fluorescein agar slant
PM-66	Galatin agar plate for gelatine hydrolysis
PM-67	G luconate peptone broth
PM-95	Hippurate broth
PM-96	KF broth detects group D strep from non D. *Also see SF broth
PM-27	Kligler's iron agar slant
PM-28	Lactose broth single strength with gas vial
PM-29	Lactose broth double strength with gas vial
PM-81	Letheen agar for environmental testing *
PM-30	Litmus milk broth tube
PM-68	Lysine iron agar slants
PM-69	Malonate broth
PM-31	Mannitol salt agar plate
PM-32	Milk agar slant
PM-33	Motility test medium
PM-34	MRVP (Methyl red-Voges-Proskauer) broth
PM-35	MacConkey agar plate
PM-36	Mueller Hinton agar plate
PM-37	Nitrate broth
PM-38	Nutrient broth
PM-39	Nutrient agar slant
PM-40	Nutrient Agar deep
PM-41	Nutrient agar plate
PM-42	Nutrient agar bottle-250 ml quantity
PM-43	Nutrient agar bottle-100 ml quantity
O-F (Oxidation-Fermentation medium with brom thymol blue (BTB) indicator for enterics*
PM-70	O-F Glucose for enterics *
PM-71	O-F Sucrose for enterics *
PM-72	O-F Lactose for enterics *
PM-97	O-F Maltose for enterics *
PM-98	O-F Mannitol for enterics *
PM-99	O-F Xylose for enterics *
O-F medium with brom cresol purple (BCP) Indicator for Staphylococci*
PM-100	O-F Glucose for Staph *
PM-101	O-F Mannitol for Staph *
PM-102	ONPG broth for beta galactosidase detection *
PM-82	Orange serum agar for lactobacilli and fungi *
PM-73	Phenylalanine agar slant
PM-44	Pyocyanin agar slant
PM-83	Photobacterium agar slant
PM-84	Selenite cystine broth enrichment broth
PM-103	SF broth detects group D enterococci from nonenterococci group D *
PM-45	Simmons citrate agar slant
PM-85	Snyder agar for oral lactobacilli *
PM-74	Sodium hippurate broth
PM-46	Spirit Blue agar plate (for detecting lipolysis - fat hydrolysis)
PM-47	SS (Salmonella-Shigella) agar plate
PM-75	Starch agar plates for starch hydrolysis test
PM-86	Tetrathionate enrichment broth
PM-48	Thioglycolate broth
PM-76	Triple sugar iron agar
PM-49	TS (Trypticase soy) broth
PM-50	TS agar slant
PM-51	TS agar deep
PM-52	TS agar plate
PM-53	TS agar bottle 250 ml quantity
PM-54	TS agar bottle 100 ml quantity
PM-55	Tryptone broth for indole test
PM-56	Urea broth $0.75 ea.
PM-87	Veillonella agar*
PM-88	XLD agar

MEDIA FOR GROWING FUNGI (MOLDS AND YEASTS) AND ACTINOMYCETES

PM-89	Actinomycete isolation agar plate
PM-77	Azide dextrose broth
PM-106	Corn meal agar slant
PM-90	Cornmeal agar plate
PM-104	Corn meal agar plates with Tween 80*
PM-57	Malt extract agar slant
PM-58	Potato dextrose agar slant
PM-59	Potato dextrose agar plate
PM-60	Sabouraud dextrose agar slant
PM-61	Sabouraud dextrose agar plate
PM-91	Trichophyton agar #4 plate *
PM-92	Trichophyton agar slant *
PM-93	Wolin Bevis agar for Candida chlamydospore production *

SPECIAL REAGENTS:

PM-94	Mineral oil - sterile for overlay	10.0 ml	$1.00 ea.
PM-105	Vaspar - sterile for overlay	2 oz	$4.50 ea

FREE CONSULTATION
If you are designing a new course or are updating an old one - call us.

We can enable you to offer a comprehensive lab course at much less of a cost than you can imagine.
Phone: 312-243-2016; FAX: 312-243-2041
e-mail:iibbt@iibbt.com

9. ANTIBIOTIC DISKS FOR SENSITIVITY TESTING

The purpose of this test, in an educational setting, is to demonstrate to the student why this test is necessary and how the test is performed. In order to impart this knowledge, a set of 4-5 different antibiotic disks is sufficient. Students can place these disks on plates, swabbed with the test organisms, with flame sterilized forceps. While multidisk dispensers are helpful, they are not essential for teaching the concept. However, if funds allow it, it is advisable to procure a dispenser so that students can see how this important test is performed routinely in hospitals.

The recommended manual method for antibiotic sensitivity testing is the Kirby-Bauer method. Laboratory handouts describing this procedure including a laboratory exercise, are available from this Institute. For ordering information, see below.

While the choice of antibiotic discs can vary, Gram positive organisms are generally tested using the following antibacterial agents:

A-01 Clindamycin 2-mcg

A-02 Lincomycin 2 mcg

A-03 Erythromycin 15 mcg

A-04 Penicillin G 10 units

A-05 Oxacillin 1-mcg or

A-06 Methicillin 5 mcg

A-07 Cephalothin 30 mcg

A-08 Tetracycline 30 mcg

A-09 Gentamicin 10 mcg

Likewise, Gram negative organisms are generally tested using the following antibacterial agents:

A-10 Ampicillin 10 mcg

A-07 Cephalothin 30 mcg

A-08 Tetracycline 30 mcg

A-09 Gentamicin 10 mcg

A-11 Carbenicillin 100 mcg

A-12 Kanamycin 30 mcg

A-13 Tobramycin 10 mcg

A-14 Amikacin 30 mcg

A-15 Nitrofurantoin 300 mcg

A-16 Trimethoprim 5 mcg or

A-17 Sulfamethoxazole 0.25 mg

Price and Ordering Information

A-18 Laboratory handout. A basic guide to the selection and use of antibiotics. Includes a laboratory exercise on sensitivity testing by the Kirby Bauer method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . $3.50

Individual vials separately containing the antibacterial agents listed above.

Price per vial . . . . . . . . . . . . . . . . .. $7.50

COST CUTTING TIP: Outdated antibiotic disks are still usable for teaching purposes; don't throw them away.

10. IMMUNOLOGICAL AND SEROLOGICAL REAGENTS

Major breakthroughs in microbiology and for that matter in biology since 1960 have been in the field of immunology and serology. Concepts and skills, especially methodology, of these important areas are, therefore, essential to providing a complete and up-to-date picture of microbiology and biology. Many of these techniques also form the basis of biotechnology and genetic engineering making their learning even more important.

Unfortunately, reagents used in these areas are produced for use by clinical laboratories and, therefore, are not only expensive, but are often hard to find. An answer to this dilemma is classroom quality immunological reagents, which are relatively inexpensive. This catalogue lists such reagents and thus opens ways for instructors to teach a variety of antigen-antibody reactions, such as the precipitin reaction, the agglutination reaction, the complement fixation reaction, the neutralization reaction, the hem agglutination reaction, the passive hem agglutination reaction, immunodouble diffusion or the Ouchterlony reaction and immunoelectrophoresis. Simultaneously, an instructor can also show how these tests are used in the design of serological tests for the diagnosis of disease, for testing the purity of proteins and other antigens and in medico legal areas such as forensics.

In addition to providing serological reagents suitable for teaching, the Institute is also available to assist you in the design and implementation of immunological and serological experiments for your classes. So, feel free to call the Institute with your questions and comments.

DETAILED LABORATORY HANDOUTS explaining the principles and procedures of immunological and serological reactions are also available from the Institute. For price information, please refer to the end of this section.

REAGENTS FOR AGGLUTINATION REACTION - with directions

Catalogue # Description Qty Price

IM-01 Killed, standardized suspension of Salmonella typhosa 5.0 ml $5.00

IM-02 Killed, standardized suspension of Salmonella Montevideo 5.0 ml $5.00

IM-03 Suspension of Salmonella typhosa marked as unknown #1 5.0 ml $5.00

IM-04 Suspension of Salmonella montevideo marked as unknown #2 5.0 ml $5.00

IM-05 Suspension of Salmonella cholerae-suis marked as unknown #3 5.0 ml $5.00

(Use of these suspensions as unknowns enables students to understand the usefulness of agglutination reactions and, also, to test their ability to read and differentiate between a positive and a negative agglutination reaction.)

IM-06 Anti Salmonella typhosa 5.0 ml $12.50

IM-07 Anti Salmonella Montevideo 5.0 ml $12.50

IM-08 Anti Salmonella cholerae-suis 5.0 ml $12.50

REAGENTS FOR PRECIPION REACTION ALSO FOR IMMUNODOUBLE DIFFUSION, ELECTROPHORESIS AND IMMUNOELECTROPHORESIS - with directions

Catalogue # Description Qty. Price

IM-09 Human serum 5.0 ml $12.50

IM-10 Goat serum 5.0 ml $7.50

IM-11 Horse serum 5.0 ml $7.50

Catalogue # Description Qty. Price

IM-12 Human gamma globulin 5.0 ml $20.00

IM-13 Human albumin 5.0 ml $10.00

IM-14 Anti human serum 5.0 ml $20.00

IM-15 Anti goat serum 5.0 ml $15.00

IM-16 Anti horse serum 5.0 ml $15.00

NOTE: For supplies for Ouchterloney double diffusion, electrophoresis and immuno-electrophoresis, refer to the Section on Special supplies and reagents for Immunological reactions.

REAGENTS FOR NEUTRALIZATION REACTION

a) Bacteriophage and antibacteriophage system - with directions

Catalogue # Description Qty. Price

VL-01 Suspension of E. coli T4 bacteriophage 2.0 ml $10.00

IM-11 Anti E. coli T4 bacteriophage 5.0 ml $10.00

LB-33 E. coli strain B $5.00

Toxin and antitoxin system - with directions

Catalogue # Description Qty. Price

IM-18 Bacterial filtrate containing hemolytic factors 3.0 ml $15.00

IM-19 Antihemolytic factors 3.0 ml $20.00

Blood plate for hemolytic reaction $1.00

REAGENTS FOR PASSIVE HEMAGGLUTINATION REACTION - with directions

Catalogue # Description Qty. Price

IM-20 Soluble antigen coupled to red blood cells 5.0 ml $25.00

IM-21 Red blood cells without soluble antigen 5.0 ml $10.00

IM-22 Anti soluble antigen 5.0 ml $15.00

IM-23 Soluble antigen solution 5.0 ml $20.00

REAGENTS FOR COMPLEMENT FIXATION REACTION - sufficient for 30 students - with directions

Catalogue # Description Qty. Price

IM-24 Antigen suspension 3.0 ml $5.00

IM-25 Positive control serum for complement fixation 5.0 ml $15.00

IM-26 Negative control serum for complement fixation 5.0 ml $10.00

IM-27 Unknown serum - for complement fixation 5.0 ml $15.00

IM-28 Complement - for complement fixation 5.0 ml $25.00

IM-29 Sheep blood for complement fixation 10.0 ml $15.00

IM-30 Anti-sheep red blood cells for complement fixation 3.0 ml $15.00

IM-31 Veronal Buffer for complement fixation 250 ml $10.00

11. REAGENTS FOR BLOOD TYPING

IM-32 Anti-A serum 5.0 ml $7.50

IM-33 Anti-B serum 5.0 ml $7.50

IM-34 Anti-Rh serum 5.0 ml $20.00